7A9Y
Structural comparison of cellular retinoic acid binding protein I and II in the presence and absence of natural and synthetic ligands
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2018-10-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 36.890, 109.980, 170.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.050 - 1.640 |
Rwork | 0.191 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cbr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.699 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.050 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.029 | 0.791 |
Rmeas | 0.029 | 0.559 |
Rpim | 0.029 | 0.559 |
Number of reflections | 43110 | 4050 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.9 | |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG mixture |