7A9Y
Structural comparison of cellular retinoic acid binding protein I and II in the presence and absence of natural and synthetic ligands
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-10-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 36.890, 109.980, 170.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.050 - 1.640 |
| Rwork | 0.191 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.050 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.029 | 0.791 |
| Rmeas | 0.029 | 0.559 |
| Rpim | 0.029 | 0.559 |
| Number of reflections | 43110 | 4050 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG mixture |
