7A9Q
Racemic compound of RNA duplexes.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.283 |
| Spacegroup name | P 1 |
| Unit cell lengths | 21.162, 25.836, 38.564 |
| Unit cell angles | 104.75, 96.56, 92.03 |
Refinement procedure
| Resolution | 37.000 - 2.185 |
| Rwork | 0.164 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zq9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.320 |
| High resolution limit [Å] | 2.185 | 2.190 |
| Rmeas | 0.185 | 0.639 |
| Number of reflections | 6865 | 1000 |
| <I/σ(I)> | 3.94 | 1.6 |
| Completeness [%] | 84.5 | 76.8 |
| Redundancy | 1.89 | |
| CC(1/2) | 0.969 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M zinc acetate, 0.1 M cacodylate buffer, 18% w/v polyethylene glycol 8000. |
