7A9D
Crystal structure of H12 Haemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.511, 104.511, 694.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 89.750 - 2.700 |
| R-factor | 0.27 |
| Rwork | 0.268 |
| R-free | 0.30310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jsd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.692 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.750 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 39909 | 5872 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 97.1 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.995 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.0, 30% Jeffamine ED2001 |
