7A99
Crystal structure of the Phe57Trp mutant of the arginine-bound form of domain 1 from TmArgBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-11 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.480, 67.217, 102.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.190 - 1.790 |
| R-factor | 0.1807 |
| Rwork | 0.178 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gpc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.841 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.190 | 1.870 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rpim | 0.033 | 0.317 |
| Number of reflections | 25021 | 3013 |
| <I/σ(I)> | 27.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium acetate trihydrate pH 4.5, 25% w/v Polyethylene glycol 3,350 |
