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7A8X

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikC with the HMA domain of Pikh-1 from rice (Oryza sativa)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04
Synchrotron siteDiamond
BeamlineI04
Temperature [K]100
Detector technologyPIXEL
Collection date2017-05-18
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.9795
Spacegroup nameP 21 21 21
Unit cell lengths65.351, 83.121, 107.842
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution65.922 - 2.300
Rwork0.210
R-free0.25590
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7a8w
RMSD bond length0.008
RMSD bond angle1.488
Data reduction softwareAimless (0.7.4)
Data scaling softwareAimless (0.7.4)
Phasing softwarePHASER (2.8.3)
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]107.840107.8402.380
High resolution limit [Å]2.3008.9102.300
Rmerge0.0610.0180.927
Rmeas0.0640.0190.968
Rpim0.0180.0060.278
Number of reflections258475432570
<I/σ(I)>22.787.12.5
Completeness [%]96.499.6100
Redundancy13.110.712
CC(1/2)1.0001.0000.864
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.52930.12 M Monosaccharides (0.2M D-Glucose; 0.2M D-Mannose; 0.2M D-Galactose; 0.2M L-Fucose; 0.2M D-Xylose; 0.2M N-Acetyl-D-Glucosamine); 0.1 M Buffer system 1 (1 M Imidazole; MES monohydrate (acid)) pH 6.5; 50% v/v Precipitant mix 1 (40% v/v PEG 500; MME; 20 % w/v PEG 20000)

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