7A8W

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikC with an engineered HMA domain of Pikp-1 from rice (Oryza sativa)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	66.784, 80.205, 105.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.165 - 2.150
Rwork	0.224
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6w
RMSD bond length	0.008
RMSD bond angle	1.479
Data scaling software	Aimless (0.6.1)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.053	0.996
Rmeas	0.055	1.034
Rpim	0.015	0.278
Number of reflections	31604	2694
<I/σ(I)>	23.9	2.3
Completeness [%]	99.9	99.8
Redundancy	13.2	13.7
CC(1/2)	1.000	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.12 M Monosaccharides (0.2M D-Glucose; 0.2M D-Mannose; 0.2M D-Galactose; 0.2M L-Fucose; 0.2M D-Xylose; 0.2M N-Acetyl-D-Glucosamine); 0.1 M Buffer system 2 (Sodium HEPES; MOPS (acid)) pH 7.5; 37.5% v/v Precipitant mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)