7A8V
Crystal structure of Polysaccharide monooxygenase from P.verruculosum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2020-04-16 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54 |
Spacegroup name | P 63 |
Unit cell lengths | 132.145, 132.145, 39.952 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.036 - 1.950 |
R-factor | 0.161874169645 |
Rwork | 0.161 |
R-free | 0.18669 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eis |
RMSD bond length | 0.007 |
RMSD bond angle | 1.013 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.040 | 2.050 |
High resolution limit [Å] | 1.940 | 1.950 |
Rmerge | 0.104 | 0.284 |
Number of reflections | 29254 | 3860 |
<I/σ(I)> | 10.28 | |
Completeness [%] | 99.0 | |
Redundancy | 4.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | cobalt chloride, MES, ammonium sulfate |