7A8N
rsGreen0.7-K206A-N205L in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | H 3 2 |
Unit cell lengths | 140.728, 140.728, 73.246 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.845 - 2.100 |
R-factor | 0.2109 |
Rwork | 0.208 |
R-free | 0.25750 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.524 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX (1.11) |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.845 | 46.840 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.057 | 0.026 | 0.713 |
Rmeas | 0.058 | 0.026 | 0.731 |
Rpim | 0.013 | 0.007 | 0.162 |
Number of reflections | 16005 | 239 | 1307 |
<I/σ(I)> | 33.1 | ||
Completeness [%] | 98.3 | 99.3 | 97.5 |
Redundancy | 20.3 | 17.1 | 20.3 |
CC(1/2) | 1.000 | 1.000 | 0.964 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES pH 7.5 25 % PEG 8000 |