7A8L

rsGreen0.7-K206A-N205C in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	140.201, 140.201, 73.124
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.709 - 1.750
R-factor	0.1901
Rwork	0.188
R-free	0.22500
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.710	46.710	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.043	0.015	0.782
Rmeas	0.045	0.016	0.817
Rpim	0.013	0.005	0.236
Total number of observations	312942
Number of reflections	27802	223	1533
<I/σ(I)>	33.1
Completeness [%]	100.0	99.4	100
Redundancy	11.3	10.6	11.7
CC(1/2)	1.000	1.000	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES pH 6.5 25 % PEG 3000