7A8L
rsGreen0.7-K206A-N205C in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 140.201, 140.201, 73.124 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.709 - 1.750 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22500 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.847 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.710 | 46.710 | 1.780 |
High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
Rmerge | 0.043 | 0.015 | 0.782 |
Rmeas | 0.045 | 0.016 | 0.817 |
Rpim | 0.013 | 0.005 | 0.236 |
Total number of observations | 312942 | ||
Number of reflections | 27802 | 223 | 1533 |
<I/σ(I)> | 33.1 | ||
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 11.3 | 10.6 | 11.7 |
CC(1/2) | 1.000 | 1.000 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.5 25 % PEG 3000 |