7A8K
rsGreen0.7-K206A-H148V partially in the green-off state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-19 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 48.006, 68.931, 76.382 |
Unit cell angles | 90.00, 94.84, 90.00 |
Refinement procedure
Resolution | 39.299 - 2.250 |
R-factor | 0.1926 |
Rwork | 0.189 |
R-free | 0.26340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xow |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.130 | 42.130 | 2.320 |
High resolution limit [Å] | 2.250 | 9.000 | 2.250 |
Rmerge | 0.076 | 0.042 | 0.697 |
Rmeas | 0.088 | 0.048 | 0.827 |
Rpim | 0.045 | 0.024 | 0.439 |
Total number of observations | 43972 | 757 | 3804 |
Number of reflections | 11804 | 201 | 1104 |
<I/σ(I)> | 10 | 26.9 | 1.9 |
Completeness [%] | 99.4 | 98.6 | 99.5 |
Redundancy | 3.7 | 3.8 | 3.4 |
CC(1/2) | 0.997 | 0.997 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM HEPES pH 7.5 12 % PEG 4000 1% tryptone |