7A8H

rsGreen0.7-K206A-H148S in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	97.593, 98.601, 100.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.512 - 1.700
R-factor	0.1807
Rwork	0.179
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.510	44.510	1.730
High resolution limit [Å]	1.700	9.310	1.700
Rmerge	0.049	0.033	0.757
Rmeas	0.051	0.036	0.788
Rpim	0.014	0.012	0.216
Total number of observations	1384862	6768	70818
Number of reflections	106573	738	5259
<I/σ(I)>	27.9	61.6	3.6
Completeness [%]	100.0	98.2	99.9
Redundancy	13	9.2	13.5
CC(1/2)	1.000	0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-tris pH 6.5 20 % PEG 5000 mme