7A8G

rsGreen0.7-K206A-H148G in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 2 21 21
Unit cell lengths	49.438, 88.622, 131.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.438 - 2.000
R-factor	0.1814
Rwork	0.179
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.915
Data reduction software	XDS
Data scaling software	Aimless (0.6.1)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.440	49.440	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.055	0.026	0.616
Rmeas	0.060	0.028	0.662
Rpim	0.022	0.011	0.239
Total number of observations	297790	2978	22425
Number of reflections	39875	530	2908
<I/σ(I)>	19.9	58.6	3.5
Completeness [%]	99.9	98.9	99.9
Redundancy	7.5	5.6	7.7
CC(1/2)	1.000	0.999	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM TMAO 100mM Tris pH 8.5 20 % Peg 2000 mme