7A8G
rsGreen0.7-K206A-H148G in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.438, 88.622, 131.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.438 - 2.000 |
R-factor | 0.1814 |
Rwork | 0.179 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.915 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.1) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.440 | 49.440 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.055 | 0.026 | 0.616 |
Rmeas | 0.060 | 0.028 | 0.662 |
Rpim | 0.022 | 0.011 | 0.239 |
Total number of observations | 297790 | 2978 | 22425 |
Number of reflections | 39875 | 530 | 2908 |
<I/σ(I)> | 19.9 | 58.6 | 3.5 |
Completeness [%] | 99.9 | 98.9 | 99.9 |
Redundancy | 7.5 | 5.6 | 7.7 |
CC(1/2) | 1.000 | 0.999 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM TMAO 100mM Tris pH 8.5 20 % Peg 2000 mme |