7A8D

rsGreen0.7-K206A-F165W partially in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	140.570, 140.570, 72.972
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.742 - 1.650
R-factor	0.1933
Rwork	0.192
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.740	46.740	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.044	0.042	0.748
Rmeas	0.046	0.045	0.785
Rpim	0.014	0.014	0.238
Total number of observations	371129	2438	17525
Number of reflections	33227	229	1624
<I/σ(I)>	27.7	80.5	3.2
Completeness [%]	100.0	99.4	100
Redundancy	11.2	10.6	10.8
CC(1/2)	0.999	0.997	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	200 mM NH4F 20 % PEG 3350