7A8C

rsGreen0.7-K206A-F145S in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	H 3 2
Unit cell lengths	140.615, 140.615, 72.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.735 - 2.130
R-factor	0.2035
Rwork	0.201
R-free	0.25270
RMSD bond length	0.009
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.730	46.730	2.190
High resolution limit [Å]	2.130	9.040	2.130
Rmerge	0.109	0.096	0.627
Rmeas	0.112	0.099	0.643
Rpim	0.025	0.024	0.143
Total number of observations	315941	3977	25612
Number of reflections	15569	229	1261
<I/σ(I)>	19.5	36.1	5.8
Completeness [%]	100.0	99.4	100
Redundancy	20.3	17.4	20.3
CC(1/2)	0.998	0.994	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES pH 6.5 25 % PEG 4000 400 mM D-galactose