7A8B
rsGreen0.7-K206A-F145S partially in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 2 |
Unit cell lengths | 139.548, 139.548, 72.864 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.513 - 1.550 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.23210 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.854 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX (1.11) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.510 | 46.510 | 1.580 |
High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
Rmerge | 0.044 | 0.030 | 0.682 |
Rmeas | 0.046 | 0.032 | 0.721 |
Rpim | 0.015 | 0.011 | 0.234 |
Total number of observations | 384350 | 2385 | 17407 |
Number of reflections | 38704 | 268 | 1885 |
<I/σ(I)> | 22.2 | 54 | 2.7 |
Completeness [%] | 98.5 | 99.4 | 97.6 |
Redundancy | 9.9 | 8.9 | 9.2 |
CC(1/2) | 0.999 | 0.999 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.0 14 % PEG 4000 |