7A8A

rsGreen0.7-K206A-F145Q partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	H 3 2
Unit cell lengths	140.760, 140.760, 73.264
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.856 - 1.930
R-factor	0.2062
Rwork	0.204
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.909
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.860	46.860	1.980
High resolution limit [Å]	1.930	9.050	1.930
Rmerge	0.052	0.035	0.670
Rmeas	0.053	0.036	0.690
Rpim	0.012	0.009	0.162
Total number of observations	419528	3743	25718
Number of reflections	20970	230	1407
<I/σ(I)>	34.1	82.3	5
Completeness [%]	100.0	99.3	100
Redundancy	20	16.3	18.3
CC(1/2)	1.000	0.998	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000