7A88
rsGreen0.7-K206A-F145M in the green-off state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.977, 139.977, 72.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.540 - 1.950 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.15) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.11) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.540 | 46.540 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.940 | 1.950 |
| Rmerge | 0.065 | 0.029 | 0.687 |
| Rmeas | 0.067 | 0.030 | 0.709 |
| Rpim | 0.016 | 0.008 | 0.174 |
| Total number of observations | 332432 | 3520 | 23052 |
| Number of reflections | 19962 | 235 | 1407 |
| <I/σ(I)> | 26.3 | 72.4 | 4.7 |
| Completeness [%] | 99.9 | 99.4 | 99.7 |
| Redundancy | 16.7 | 15 | 16.4 |
| CC(1/2) | 1.000 | 1.000 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MMT 25 % PEG 1500 |
