7A87

rsGreen0.7-K206A-F145M in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	139.313, 139.313, 72.455
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.360 - 1.750
R-factor	0.1934
Rwork	0.192
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.360	46.360	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.052	0.027	0.699
Rmeas	0.055	0.029	0.734
Rpim	0.017	0.009	0.222
Total number of observations	276606	2118	15638
Number of reflections	27188	217	1478
<I/σ(I)>	25.8	74.9	3.8
Completeness [%]	100.0	99.4	99.8
Redundancy	10.2	9.8	10.6
CC(1/2)	1.000	1.000	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-tris pH 6.5 20 % PEG 1500