7A85
rsGreen0.7-K206A-F145L in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 46.303, 60.910, 92.693 |
Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
Resolution | 43.241 - 1.520 |
R-factor | 0.2129 |
Rwork | 0.212 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.879 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.23) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.240 | 43.240 | 1.550 |
High resolution limit [Å] | 1.520 | 8.330 | 1.520 |
Rmerge | 0.051 | 0.031 | 0.606 |
Rmeas | 0.059 | 0.036 | 0.712 |
Rpim | 0.030 | 0.017 | 0.367 |
Total number of observations | 146055 | 1023 | 7044 |
Number of reflections | 39087 | 259 | 1909 |
<I/σ(I)> | 12.4 | 34.8 | 1.7 |
Completeness [%] | 99.2 | 98.7 | 99.4 |
Redundancy | 3.7 | 3.9 | 3.7 |
CC(1/2) | 0.999 | 0.998 | 0.803 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.5 12 % PEG 20 000 10 mM NaBr |