7A84
rsGreen0.7-K206A-F145H partially in the green-off state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.973, 139.973, 73.236 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.693 - 2.100 |
| R-factor | 0.2233 |
| Rwork | 0.221 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.690 | 46.690 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.046 | 0.028 | 0.768 |
| Rmeas | 0.047 | 0.029 | 0.786 |
| Rpim | 0.010 | 0.007 | 0.168 |
| Total number of observations | 323440 | 3839 | 28112 |
| Number of reflections | 15971 | 236 | 1294 |
| <I/σ(I)> | 36.8 | 97 | 5 |
| Completeness [%] | 99.1 | 98.6 | 98.3 |
| Redundancy | 20.3 | 16.3 | 21.7 |
| CC(1/2) | 1.000 | 1.000 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.0 14 % PEG 400 300 mM NGSB-195 |
