7A83
rsGreen0.7-K206A-F145H in the green-on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 45.750, 61.135, 91.831 |
| Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
| Resolution | 42.817 - 1.730 |
| R-factor | 0.1657 |
| Rwork | 0.164 |
| R-free | 0.19240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.937 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.820 | 42.820 | 1.760 |
| High resolution limit [Å] | 1.730 | 8.990 | 1.730 |
| Rmerge | 0.044 | 0.030 | 0.682 |
| Rmeas | 0.048 | 0.032 | 0.736 |
| Rpim | 0.018 | 0.013 | 0.275 |
| Total number of observations | 178548 | 1190 | 10045 |
| Number of reflections | 25749 | 201 | 1406 |
| <I/σ(I)> | 21 | 49.1 | 2.8 |
| Completeness [%] | 98.1 | 97.6 | 97 |
| Redundancy | 6.9 | 5.9 | 7.1 |
| CC(1/2) | 0.999 | 0.999 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 125 mM MES pH 6.0 9 % PEG 4000 |
