7A82

rsGreen0.7-K206A-F145A partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	H 3 2
Unit cell lengths	141.363, 141.363, 73.108
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.808 - 1.900
R-factor	0.2016
Rwork	0.199
R-free	0.24990
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.930	46.930	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.045	0.034	0.774
Rmeas	0.046	0.035	0.793
Rpim	0.010	0.009	0.176
Total number of observations	445440	3243	29009
Number of reflections	22123	223	1410
<I/σ(I)>	37.7	92.3	4.5
Completeness [%]	100.0	99.1	100
Redundancy	20.1	14.5	20.6
CC(1/2)	1.000	0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000 200 mM NDSB-211