7A80

rsGreen0.7-K206A-E222V in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	69.822, 61.800, 112.121
Unit cell angles	90.00, 91.01, 90.00

Refinement procedure

Resolution	46.274 - 2.050
R-factor	0.1854
Rwork	0.183
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.270	46.270	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.097	0.049	0.682
Rmeas	0.105	0.054	0.739
Rpim	0.041	0.022	0.282
Total number of observations	405037	3998	30415
Number of reflections	60229	715	4424
<I/σ(I)>	13.2	28	2.9
Completeness [%]	99.9	98.3	100
Redundancy	6.7	5.6	6.9
CC(1/2)	0.998	0.997	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	26 % PEG 4000 500 mM Mg(NO3) 50 mM HEPES pH 7.5