7A7Z
rsGreen0.7-K206A-E222G in the green-on state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 48.245, 61.556, 93.098 |
| Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
| Resolution | 40.402 - 1.100 |
| R-factor | 0.1235 |
| Rwork | 0.122 |
| R-free | 0.14240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.034 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.910 | 44.910 | 1.120 |
| High resolution limit [Å] | 1.100 | 6.020 | 1.100 |
| Rmerge | 0.029 | 0.017 | 0.623 |
| Rmeas | 0.033 | 0.019 | 0.740 |
| Rpim | 0.017 | 0.009 | 0.392 |
| Total number of observations | 2737 | 18148 | |
| Number of reflections | 108890 | 700 | 5350 |
| <I/σ(I)> | 16.7 | 59.4 | 1.8 |
| Completeness [%] | 99.6 | 99.1 | 99.7 |
| Redundancy | 3.6 | 3.9 | 3.4 |
| CC(1/2) | 1.000 | 0.999 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM (Li)2SO4 100 mM Tris pH 8.5 40 % PEG 400 |
