7A7Z

rsGreen0.7-K206A-E222G in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	I 1 2 1
Unit cell lengths	48.245, 61.556, 93.098
Unit cell angles	90.00, 97.41, 90.00

Refinement procedure

Resolution	40.402 - 1.100
R-factor	0.1235
Rwork	0.122
R-free	0.14240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.034
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.910	44.910	1.120
High resolution limit [Å]	1.100	6.020	1.100
Rmerge	0.029	0.017	0.623
Rmeas	0.033	0.019	0.740
Rpim	0.017	0.009	0.392
Total number of observations		2737	18148
Number of reflections	108890	700	5350
<I/σ(I)>	16.7	59.4	1.8
Completeness [%]	99.6	99.1	99.7
Redundancy	3.6	3.9	3.4
CC(1/2)	1.000	0.999	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM (Li)2SO4 100 mM Tris pH 8.5 40 % PEG 400