7A7X
rsGreen0.7-F145M in the green-off state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.916, 62.084, 106.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.408 - 1.850 |
R-factor | 0.1503 |
Rwork | 0.147 |
R-free | 0.20700 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.877 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHENIX (1.11) |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.410 | 40.410 | 1.890 |
High resolution limit [Å] | 1.850 | 9.060 | 1.850 |
Rmerge | 0.084 | 0.041 | 0.644 |
Rmeas | 0.088 | 0.044 | 0.672 |
Rpim | 0.025 | 0.014 | 0.191 |
Number of reflections | 18843 | 208 | 1160 |
<I/σ(I)> | 19.1 | ||
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 12.5 | 9.2 | 12.6 |
CC(1/2) | 0.999 | 0.999 | 0.934 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MMT pH 8.0 25 % PEG 1500 |