7A7S
rsGreenF in the green-on state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.333, 61.968, 46.779 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 46.758 - 1.730 |
| R-factor | 0.2019 |
| Rwork | 0.200 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xow |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.766 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.760 | 46.760 | 1.760 |
| High resolution limit [Å] | 1.730 | 8.990 | 1.730 |
| Rmerge | 0.058 | 0.044 | 0.631 |
| Rmeas | 0.063 | 0.049 | 0.685 |
| Rpim | 0.024 | 0.021 | 0.263 |
| Total number of observations | 147910 | ||
| Number of reflections | 22182 | 175 | 1195 |
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 99.4 | 99.4 | 99.1 |
| Redundancy | 6.7 | 5.9 | 6.7 |
| CC(1/2) | 0.998 | 0.996 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MIB pH 4.0 25 % PEG 1500 |
