7A7Q

rsGreenF-K206A in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	106.313, 106.313, 35.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.545 - 2.000
R-factor	0.1642
Rwork	0.161
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.545	47.540	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.083	0.037	0.768
Rmeas	0.085	0.038	0.783
Rpim	0.017	0.009	0.152
Number of reflections	14432	206	1040
<I/σ(I)>	26.6
Completeness [%]	100.0	99.6	100
Redundancy	25.1	18.6	26.5
CC(1/2)	0.999	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000