7A7P

rsGreen0.7-K206A partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 2 21 21
Unit cell lengths	50.808, 61.352, 65.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.930 - 1.480
R-factor	0.1711
Rwork	0.170
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.930	44.930	1.510
High resolution limit [Å]	1.480	8.110	1.480
Rmerge	0.059	0.043	0.689
Rmeas	0.064	0.049	0.747
Rpim	0.025	0.022	0.283
Total number of observations	240614
Number of reflections	35063	259	1716
<I/σ(I)>	15.1
Completeness [%]	99.8	97.8	100
Redundancy	6.9	4.7	7.1
CC(1/2)	0.998	0.994	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22 % PEG 4000 140 mM Mg(NO3)2