7A7O

rsGreen0.7-K206A in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	140.209, 140.209, 72.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.838 - 1.800
R-factor	0.1975
Rwork	0.195
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.988
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.650	46.650	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.051	0.027	0.730
Rmeas	0.054	0.029	0.779
Rpim	0.019	0.010	0.270
Total number of observations	212503
Number of reflections	25487	229	1509
<I/σ(I)>	24.6
Completeness [%]	100.0	98.9	100
Redundancy	8.3	8	8.2
CC(1/2)	1.000	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM Ammonium acetate 100 mM Bis-tris pH 5.5 25 % PEG 3350