7A7L

rsEGFP in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	51.108, 62.003, 71.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.760 - 1.300
R-factor	0.1467
Rwork	0.145
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.760	46.760	1.320
High resolution limit [Å]	1.300	7.120	1.300
Rmerge	0.121	0.111	0.702
Rmeas	0.138	0.129	0.803
Rpim	0.064	0.065	0.382
Total number of observations	250702
Number of reflections	56215	406	2726
<I/σ(I)>	6.8
Completeness [%]	99.8	99.1	98.8
Redundancy	4.5	4	4.2
CC(1/2)	0.985	0.959	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.0 M (NH4)2SO4 200 mM K/Na-tartrate 100 mM citrate pH 5.0 100 mM b-Nicotinamide adenine dinucleotide