7A7K
rsEGFP in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.477, 51.400, 47.622 |
Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
Resolution | 47.186 - 1.550 |
R-factor | 0.1706 |
Rwork | 0.169 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eul |
RMSD bond length | 0.006 |
RMSD bond angle | 0.883 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.7) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.190 | 47.190 | 1.580 |
High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
Rmerge | 0.067 | 0.048 | 0.751 |
Rmeas | 0.079 | 0.056 | 0.876 |
Rpim | 0.040 | 0.028 | 0.444 |
Total number of observations | 122879 | ||
Number of reflections | 33023 | 223 | 1610 |
<I/σ(I)> | 10.4 | ||
Completeness [%] | 99.7 | 99.2 | 99.9 |
Redundancy | 3.7 | 3.4 | 3.8 |
CC(1/2) | 0.997 | 0.996 | 0.558 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM (NH4)SO4 100 mM Bis-trip pH 5.5 25 % PEG 3350 |