7A73
Crystal structure of the two-domain cyclophilinA from Anabaena sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.120, 103.060, 44.170 |
Unit cell angles | 90.00, 114.74, 90.00 |
Refinement procedure
Resolution | 37.380 - 1.140 |
R-factor | 0.1801 |
Rwork | 0.179 |
R-free | 0.19810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rfy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.104 |
Data reduction software | DIALS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.380 | 1.180 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.200 | 1.800 |
Rmeas | 0.200 | 1.000 |
Rpim | 0.040 | 1.000 |
Number of reflections | 115078 | 11028 |
<I/σ(I)> | 8.1 | 0.4 |
Completeness [%] | 95.0 | 91.14 |
Redundancy | 19.8 | |
CC(1/2) | 0.993 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200mM ammonium citrate 20% PEG 3350 10% Morpheus additive screen |