7A6P
Structural determinants underlying the adduct lifetime in a short LOV protein PpSB2-LOV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.142, 88.467, 54.180 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 44.880 - 1.930 |
| R-factor | 0.1777 |
| Rwork | 0.177 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sw1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.880 | 44.880 | 1.980 |
| High resolution limit [Å] | 1.930 | 9.050 | 1.930 |
| Rmerge | 0.097 | 0.039 | 0.890 |
| Rmeas | 0.111 | 0.045 | |
| Rpim | 0.053 | 0.022 | 0.508 |
| Total number of observations | 107111 | ||
| Number of reflections | 25353 | 259 | 1705 |
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 4.2 | 4.2 | 4 |
| CC(1/2) | 0.997 | 0.997 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 292.15 | Sodium acetate, polyethylene glycol 200, polyethylene glycol 3350 |
