7A5E

OSM-3 kinesin motor domain complexed with Mg.AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	C 2 2 21
Unit cell lengths	100.030, 102.200, 140.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.040 - 1.904
R-factor	0.2077
Rwork	0.206
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a3z
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.040	1.950
High resolution limit [Å]	1.904	1.904
Rmeas	0.098
Number of reflections	56649	8937
<I/σ(I)>	13.7	0.8
Completeness [%]	99.8
Redundancy	13.6
CC(1/2)	0.999	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% (W/V) polyethylene glycol 3350, 170 mM (NH4)2SO4, and 0.1 M Mes buffer pH 6.5