7A57
La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 170.873, 170.873, 379.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.057 - 3.155 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7a56 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.924 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.173 | 3.485 |
High resolution limit [Å] | 3.155 | 3.155 |
Rmerge | 0.132 | 1.076 |
Rmeas | 0.144 | 1.177 |
Rpim | 0.057 | 0.470 |
Number of reflections | 38252 | 1913 |
<I/σ(I)> | 9.2 | 1.8 |
Completeness [%] | 67.1 | 13.3 |
Redundancy | 6.3 | 6 |
CC(1/2) | 0.998 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.2 uL of 8.5 mg/mL trimeric LACV Gc(918-1364) W1066H in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.2 uL of reservoir solution containing 0.1 M Bis-Tris propane pH 7 and 1.2 M K/Na tartrate (final pH: 8.0) |