7A56

Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-29
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.8729
Spacegroup name	H 3 2
Unit cell lengths	86.790, 86.790, 358.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.407 - 1.850
R-factor	0.1717
Rwork	0.170
R-free	0.20850
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.362
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.407	43.407	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.105	0.062	1.094
Rmeas	0.115	0.067	1.188
Number of reflections	44636	560	3225
<I/σ(I)>	9.7	26.65	1.8
Completeness [%]	99.2	94.4	98.8
Redundancy	6.232	5.714	6.503
CC(1/2)	0.997	0.996	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291.15	0.5 uL of 4.5 mg/mL trimeric SBV Gc fusion domain fragment in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.5 uL of reservoir solution containing 20% w/v PEG 1K, 0.1 M Na/K phosphate pH 6.2 and 0.2 M NaCl