7A50

Crystal structure of the APH coiled-coil in complex with nanobody Nb26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978570
Spacegroup name	H 3 2
Unit cell lengths	198.220, 198.220, 58.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.320 - 1.999
R-factor	0.1843
Rwork	0.182
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modeler
RMSD bond length	0.009
RMSD bond angle	1.001
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.320	2.071
High resolution limit [Å]	1.999	1.999
Rmeas	0.222
Number of reflections	29399	2821
<I/σ(I)>	8.07	1.39
Completeness [%]	99.5	95.85
Redundancy	2.07	3.22
CC(1/2)	0.991	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	0.2 M calcium chloride, 0.1 M sodium acetate pH 5, 20 % w/v PEG 6000