7A4Y

Crystal structure of the P5P6 coiled-coil in complex with nanobody Nb34.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980126
Spacegroup name	P 32 2 1
Unit cell lengths	72.450, 72.450, 193.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.008 - 2.157
R-factor	0.1977
Rwork	0.196
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modeler
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.008	2.234
High resolution limit [Å]	2.157	2.157
Rmeas	0.122
Number of reflections	32518	3060
<I/σ(I)>	14.99
Completeness [%]	99.4	95.4
Redundancy	11
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.2 M ammonium sulphate, Bis-Tris pH 5.5, 25% PEG 3350