7A4X
tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant in complex with dimethyl sulfoxide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.79990 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 128.122, 128.122, 114.408 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.060 - 2.050 |
R-factor | 0.1726 |
Rwork | 0.171 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.007 |
RMSD bond angle | 0.794 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.060 | 2.170 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 35368 | 5626 |
<I/σ(I)> | 22.3 | 5.7 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 21.9 | |
CC(1/2) | 0.999 | 0.957 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.1 M TRIS, pH 8.5, 5% PEG 6000 |