7A4U
Crystal structure of lid-truncated apo BiP in an oligomeric state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9760 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.333, 51.289, 119.716 |
| Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
| Resolution | 58.910 - 1.770 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hab |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.341 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.920 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 58872 | 3347 |
| <I/σ(I)> | 9.9 | 1.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.2 | |
| CC(1/2) | 0.975 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M BIS-TRIS propane pH 7.5, 0.2 M trisodium citrate dihydrate, 20% PEG 3350 |
