7A4T

Crystal structure of the GCN coiled-coil in complex with nanobody Nb39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980127
Spacegroup name	P 42 3 2
Unit cell lengths	106.640, 106.640, 106.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.691 - 2.124
R-factor	0.1882
Rwork	0.185
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modeler
RMSD bond length	0.008
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.691	2.201
High resolution limit [Å]	2.124	2.125
Rmeas	0.121
Number of reflections	12227	1177
<I/σ(I)>	27.45
Completeness [%]	99.9	99.75
Redundancy	20
CC(1/2)	1.000	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M sodium formate 20 % w/v PEG 3350