7A4D

Crystal structure of the APH coiled-coil in complex with nanobodies Nb28 and Nb30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978570
Spacegroup name	P 21 21 21
Unit cell lengths	61.260, 127.450, 192.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.227 - 2.694
R-factor	0.1789
Rwork	0.176
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modeler
RMSD bond length	0.009
RMSD bond angle	1.128
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.227	2.791
High resolution limit [Å]	2.694	2.694
Rmeas	0.144	1.516
Number of reflections	42539	4077
<I/σ(I)>	15.52
Completeness [%]	99.5	95.74
Redundancy	11.82
CC(1/2)	0.998	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.2 M sodium chloride, 2.0 M ammonium sulfate, 0.1 M Sodium cacodylate 6.5