7A40
Nucleotide-free OSM-3 kinesin motor domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979338 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.770, 155.180, 63.720 |
Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
Resolution | 47.590 - 2.297 |
R-factor | 0.2265 |
Rwork | 0.225 |
R-free | 0.25390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7a3z |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 2.400 |
High resolution limit [Å] | 2.297 | 2.300 |
Rmeas | 0.121 | |
Number of reflections | 34809 | 5500 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 99.6 | 95.4 |
Redundancy | 7 | 6.6 |
CC(1/2) | 0.997 | 0.625 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 20% (W/V) polyethylene glycol 3350, 170 mM (NH4)2SO4, and 0.1 M Mes buffer at pH 6.5 |