7A3R
Crystal structure of dengue 1 virus envelope glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-07-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 105.190, 105.190, 180.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 3.600 |
R-factor | 0.2534 |
Rwork | 0.250 |
R-free | 0.29540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uzg |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 3.700 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.219 | 0.476 |
Number of reflections | 13279 | 1797 |
<I/σ(I)> | 9.9 | |
Completeness [%] | 98.7 | 94.7 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 50 mM Tris pH 8.0, 18% PEG 3,350, 400 mM Mg Acetate, 200 mM NaCl |