7A1V

Crystal structure of YTHDF2 in complex with m1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	79.781, 79.781, 113.563
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.870 - 2.200
R-factor	0.2232
Rwork	0.222
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wqn
RMSD bond length	0.003
RMSD bond angle	0.485
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.870	3.800	2.330
High resolution limit [Å]	2.200	3.300	2.200
Rmeas	0.041	0.033	0.640
Number of reflections	40931	4262	6577
<I/σ(I)>	31	57.29	3.01
Completeness [%]	100.0	100	99.4
Redundancy	7.1
CC(1/2)	0.999	0.999	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277.15	0.1M Sodium Citrate Tribasic Dihydrate pH 5.2, 2 M ammonium sulfate.