7A1V
Crystal structure of YTHDF2 in complex with m1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 79.781, 79.781, 113.563 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.870 - 2.200 |
R-factor | 0.2232 |
Rwork | 0.222 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wqn |
RMSD bond length | 0.003 |
RMSD bond angle | 0.485 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.870 | 3.800 | 2.330 |
High resolution limit [Å] | 2.200 | 3.300 | 2.200 |
Rmeas | 0.041 | 0.033 | 0.640 |
Number of reflections | 40931 | 4262 | 6577 |
<I/σ(I)> | 31 | 57.29 | 3.01 |
Completeness [%] | 100.0 | 100 | 99.4 |
Redundancy | 7.1 | ||
CC(1/2) | 0.999 | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277.15 | 0.1M Sodium Citrate Tribasic Dihydrate pH 5.2, 2 M ammonium sulfate. |