7A1R

Crystal structure of the C2B domain of Trypanosoma brucei extended synaptotagmin (E-Syt)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-09-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	54.908, 57.532, 84.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 1.500
R-factor	0.1705
Rwork	0.170
R-free	0.19070
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.083	0.978
Rmeas	0.092	1.035
Rpim	0.039	0.429
Number of reflections	43547	4297
<I/σ(I)>	10.39	1.37
Completeness [%]	99.6	99.8
Redundancy	5.4	5.5
CC(1/2)	0.998	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M sodium cacodylate (pH 6.5), 0.15 M sodium acetate, 28% (v/v) polyethylene glycol 8,000