7A0K
Crystal structure of the entire ectodomain from the Physcomitrella patens receptor kinase CR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-11 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999894 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.579, 183.962, 98.173 |
Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
Resolution | 45.870 - 2.700 |
R-factor | 0.2174 |
Rwork | 0.216 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7a0j |
RMSD bond length | 0.003 |
RMSD bond angle | 0.594 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.870 | 2.860 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmeas | 0.151 | 1.890 |
Number of reflections | 85660 | 13775 |
<I/σ(I)> | 12 | 1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7 | 6.6 |
CC(1/2) | 0.998 | 0.464 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15 % PEG 4,000, 0.2 M imidazole malate pH 7.0 |