7A0H

Structure of homodimeric actin capping protein alpha subunit from Plasmodium berghei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	C 1 2 1
Unit cell lengths	164.490, 35.168, 115.438
Unit cell angles	90.00, 104.44, 90.00

Refinement procedure

Resolution	74.200 - 2.220
R-factor	0.2095
Rwork	0.207
R-free	0.25680
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.474
Data reduction software	DIALS (1.10.4)
Data scaling software	xia2 (0.5.582)
Phasing software	HKL2Map (0.4c-beta)
Refinement software	PHENIX (dev-3928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.650	2.299
High resolution limit [Å]	2.220	2.220
Rmerge	0.260	4.372
Rmeas	0.263	4.657
Rpim	0.040	1.571
Number of reflections	61855	6109
<I/σ(I)>	10.62	0.45
Completeness [%]	99.2	95.57
Redundancy	40.7	8.4
CC(1/2)	0.999	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1M MES, 0.133 M triammonium-citrate, 10-12% (w/v) PEG 20k, 0.5 M NDSB-195