7A0A
Crystal structure of mouse CSAD in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.060, 114.880, 113.760 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 47.020 - 2.800 |
| R-factor | 0.2449 |
| Rwork | 0.243 |
| R-free | 0.29170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jis |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.612 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc7_3834) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.504 | 3.809 |
| Number of reflections | 45520 | 3321 |
| <I/σ(I)> | 3.4 | 0.4 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.949 | 0.184 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 200 mM sodium sulphate, 100 mM bis-tris propane, 20% PEG3350, pH 6.5 |
