7ZXF

Pfs48/45 bound to monoclonal antibodies 10D8 and 85RF45.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.99998
Spacegroup name	P 41 21 2
Unit cell lengths	156.880, 156.880, 148.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.160 - 3.720
R-factor	0.2817
Rwork	0.281
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h5n
RMSD bond length	0.003
RMSD bond angle	0.600
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.000	4.060
High resolution limit [Å]	3.720	3.720
Rpim	0.027	0.875
Number of reflections	456518	56247
<I/σ(I)>	15.7
Completeness [%]	94.9
Redundancy	26.8
CC(1/2)	0.999	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Lithium sulfate, 0.1M HEPES pH 7.0 and 20 % w/v Polyvinylpyrrolidone